Department: Biochemistry

E-mail: tim@biochem.utah.edu
Address: 5520 EEJ
Phone: 581-5435

Research Title: Yeast Saccharomyces cerevisiae as a model to study how the structure of chromatin influences DNA Replication and Transcription

Research Description:

My lab uses the yeast Saccharomyces cerevisiae as a model to study how the structure of chromatin influences DNA Replication and Transcription. We currently focus on the properties of a complex called FACT that allows regulatory factors and polymerases to access DNA that is otherwise hidden within nucleosomes, the basic subunit of chromatin. FACT is an essential factor in human cells but using a model system to study FACT allows us to ask detailed questions that cannot be answered easily with human cells. Approaches include analyzing living yeast cells by genetic methods, studying the properties of purified components through in vitro biochemistry, and determining the structure of FACT components by x-ray crystallography through a collaboration with Chris Hill. A typical project involves introducing a mutation into a gene encoding a FACT subunit into the yeast genome, studying the defects this causes in replication or transcription, identifying secondary mutations that make this defect more tolerable or less tolerable, then purifying the components and measuring their enzymatic properties in vitro. This gives practical experience with Molecular Biology, Genetics, and Biochemistry, as well as exposure to the principles of scientific inquiry, within a short time frame.

Does this research involve human subjects or animals?

If yes, what is the protocol number?

1/2008