| Kimble Frazer, Ph.D. MD
E-mail: kimble.frazer@hsc.utah.edu Changes to the DNA methylation and transcription patterns that accompany lymphocyte commitment to the B and T cell lineages. |
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A study investigating changes to the DNA methylation and transcription patterns that accompany lymphocyte commitment to the B and T cell lineages. Cell populations being studied are immature B cells, mature B cells, immature T lymphocytes (thymocytes), and mature T cells. Lymphocytes are obtained by dissections of thymus and marrow from adult fish, followed by fluorescence-activated cell sorting (FACS). Genomic DNA and total RNA are then purified. To isolate the methylated DNA in the genome, immunoprecipitation using an anti-5’-methylcytosine antibody is then performed. Finally, immunoprecipitated methyl-DNA and total RNA fractions are then hybridized to microarrays containing representations of the entire zebrafish genome. Using this strategy, we hope to identify genes with key roles governing the choices between T versus B lineage, as well as immature vs. mature lymphocyte. Students participating in this project can expect to be involved in any and all steps of the procedure described above, from zebrafish dissections to microarray analysis. 1/2008 |
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