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Structural and mechanistic questions related to proteasome activation, HIV assembly and budding, and nucleosome reorganization and remodeling. In all cases, central approaches include expression of proteins from recombinant sources in bacteria, yeast, or insect cells. Purified proteins are characterized hydrodynamically and for their binding interactions. Relevant proteins and complexes are subject to crystallization trials and structures are determined by x-ray crystallography or in the case of some relatively small proteins by nuclear magnetic resonance (NMR). The structural studies typically inform mutagenic/biochemical experiments to test ideas of mechanism and biological studies, which may be performed as a collaboration, to understand the biological importance and context of the biochemical activities. 1/2008 |
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